The natural composition of our products

Many ask what our products contain. Besides bacteria like the Bacillus Subtilis and enzymes, they naturally include surfactin. This biosurfactant helps break down organic compounds, can have anti-tumor effects, and supports immune regulation. Below is some information about these widespread natural molecules.

Molecular composition of Surfactin

Biosurfactant in nature

Biosurfactants are natural molecules made by microbes that have both water- and oil-attracting parts. They lower water’s surface tension and help mix (emulsify) oils. Most are glycolipids made by bacteria that eat or break down insoluble organic matter.

They help break down pollutants and bind contaminants. Microbes produce them where non-soluble organics are present, and production can be increased by using specific culture media, surface-tension tests, and molecular methods.

Biosurfactants come in two main types:

  • Low molecular weight (LMW): glycolipids and lipopeptides, which are good at reducing surface tension.

  • High molecular weight (HMW): polymeric substances (polysaccharides, proteins, lipoproteins) that stick to surfaces and act as bioemulsifiers.

Examples: trehalolipids (from Nocardia, Mycobacterium, Corynebacterium) with possible antitumor and immune effects; surfactin (from Bacillus) with antitumor properties and potential for oral insulin delivery.

Advantages over synthetic surfactants include lower toxicity, better biodegradability, and effectiveness across varied temperatures and conditions.

Bacillus Subtilis

Bacillus subtilis (/bəˈsɪl.əs subˈtiː.lis/),[3][4] known also as the hay bacillus or grass bacillus, is a gram-positive, catalase-positive bacterium, found in soil and the gastrointestinal tract of ruminants, humans and marine sponges.[5][6][7][8] Bacillus subtilis is motile and amylase positive.[9][10] It forms biofilms through the formation of extracellular polymeric matrix containing sugars and proteins.[11] As a member of the genus Bacillus, B. subtilis is rod-shaped, and can form a tough, protective endospore, allowing it to tolerate extreme environmental conditions. B. subtilis has historically been classified as an obligate aerobe, though evidence exists that it is a facultative anaerobe. B. subtilis is considered the best studied Gram-positive bacterium and a model organism to study bacterial chromosome replication and cell differentiation. It is one of the bacterial champions in secreted enzyme production and used on an industrial scale by biotechnology companies.[5][6][7]

Sources:

1: https://medcraveonline.com/JBMOA/role-of-biosurfactants-in-nature-and-biotechnological-applications.html

2: https://www.sciencedirect.com/science/article/abs/pii/S0001868619302544

3: https://www.nature.com/articles/s41598-020-58330-x

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